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NG2-glia proliferation and differentiation following CNS injuries
Kirdajová, Denisa ; Anděrová, Miroslava (advisor) ; Procházka, Jan (referee) ; Novák, Ondřej (referee)
NG2-glia proliferation and differentiation following CNS injuries Abstract NG2 glia display wide proliferation and differentiation potential under physiological and pathological conditions. They are very well known as precursors of oligodendrocytes, however, following central nervous system (CNS) injury they play an important role in regeneration. For this reason, we examined these features following different types of brain disorders such as focal cerebral ischemia (FCI), cortical stab wound (SW), and demyelination (DEMY) in young (3-months-old) mice, in which NG2 glia are labeled by tdTomato under the Cspg4 promoter. In the case of FCI, the factor of age was also studied using 18-months-old mice. To address these issues, we employed many techniques on tissue/cellular levels, such as single-cell RT- qPCR, single-cell/bulk RNA-sequencing, immunohistochemistry, and the patch-clamp technique in situ. First, such approach enabled us to distinguish two main populations (NG2 glia, oligodendrocytes), each of them comprising four distinct subpopulations. Next, the expression profiling revealed that a subpopulation of NG2 glia expressing GFAP, a marker of reactive astrocytes, appears transiently after FCI. However, following less severe injury, namely the cortical SW and DEMY, subpopulations mirroring different...
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Case study of physiotherapy treatment of a patient with the diagnosis multiple sclerosis.
Valková, Barbora ; Novotná, Irena (advisor) ; Křížková, Štěpánka (referee)
Title: Case study of physiotherapy treatment of a patient with the diagnosis multiple sclerosis. Goals of assignment: Goal of common part of assignment was through study specialized literature regarding multiple sclerosis and it is etiology, prevalence of disease, diagnostic, types of treatment methods and procedures of physiotherapy. In special part was goal processing patient's casuistry his illness, application theoretical skills methods of physiotherapy and follow - up effect executed therapy. Methodology of assignment: Bachelor assignment is concentrated on physiotherapeutic treatment of patient with multiple sclerosis. In common part is described briefly anatomy and kinesiology which is pertinent to illness. In characterization of illness I deal with the probably etiology prevalence, individual types of illness, clinical image and factors which can affect illness. I devote my attention to diagnostic, prognosis and treatment. The treatment is divides into pharmacological, alternative and physiotherapeutic ones. The special part is written in the form of casuistry of patient with diagnosis multiple sclerosis. After the incoming kineziological assessment, long - term and short - term, therapeutic plan, process of single therapeutic units which include goal of the therapeutic unit, suggestion of...
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SUBJECTIVE VIEW ON QUALITY OF LIFE OF PEOPLE WITH MULTIPLE SCLEROSIS AT SAINT JOSEF´S HOUSE AT ŽIREČ.
KRÁLOVÁ, Inka
Sclerosis multiplex (disseminated) is a chronic inflammatory disease of the central nervous system occuring to young people mostly. This is a diagnosis that changes a person{\crq}s life entirely. The people suffering from the disease gradually become fully dependent on the other people{\crq}s help. The disease is usually diagnosed at people at the age 20 {--} 40. Domov sv. Josefa (St. Joseph{\crq}s Hospice) in Žireč provides good conditions for dignified and full-value life of the ill people, and psychical support in their fight with the destiny. The hospice is the only of its kind in the Czech Republic though the number of applications for staying here is increasing and the capacity of the hospice is not sufficient. Most people with the diagnosis of sclerosis multiplex stay in seclusion; mostly they get divorced or stay with their elderly parents. Domov sv. Josefa offers the ill people the solution of their difficult life situation. In my work I found out patients{\crq} subjective opinions on the quality of their lives in the hospice and how the people with sclerosis disseminated live in the ward of permanent residence in Domov sv. Josefa. The essay should help to increase the awareness of Domov sv. Josefa because I believe the care facilities of the kind should be more numerous in the Czech Republic. This is a way how to solve the difficult situation of many people suffering from sclerosis disseminated and how to allow them to have dignified, active and full-value lives.
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